CPT tube 8ml

Tlhaloso e Khutšoanyane:

Mohlala No.	CPT08
Lintho tse bonahalang	Khalase e sa jeleng paate ea Pharmaceutical
Keketso	Anticoagulant + Gel + Ficoll Tharollo
Kopo	Ho ntšoa ha lisele tsa Mononuclear bakeng sa lipatlisiso
Boholo ba Tube	16 * 120mm
Thala Bolumo	8ML
Tekanyetso ea polokeho	Maemo a Tšireletso a Machaba
Lihlopha tsa ho rekisa	200 li-tubes / lebokose
Mobala oa Cap	Botšo & Boputsoa
Mohlala	E fumaneha
Shelf Life	1 Lilemo
OEM / ODM	Label, thepa, moralo oa liphutheloana lia fumaneha.
Setifikeiti sa Boleng	ISO13485, FSC, MDSAP
Express	DHL, FedEx, TNT, UPS, EMS, SF, joalo-joalo.
Tefo	L/C, T/T, Western Union, Paypal, joalo-joalo.

Sephutheloana

Sephutheloana sa motho ka mong ka mokotleng oa pampiri;

Re romelle lengolo-tsoibila

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

A Vacutainer CPT (Cell Preparation Tube) 8mL ke tšupu e khethehileng ea pokello ea mali e sebelisetsoang ho arola lisele tsa mononuclear (PBMCs) maling a felletseng. E sebelisoa hangata ho immunology, lipatlisiso tsa mafu a tšoaetsanoang, le lits'ebetso tsa bongaka tse kang tlhahlobo ea 'mele ea ho itšireletsa mafung le liteko tse thehiloeng liseleng.

Lintlha tsa bohlokoa tsa Vacutainer CPT Tube (8mL):
E tlatsitsoe esale pele ka anticoagulant le density gradient medium
E na le Sodium Citrate joalo ka anticoagulant.
E kenyelletsa gel ea karohano ea Ficoll™-based density gradient ho thusa karohano ea lisele tsa mononuclear.
E nolofalletsa PBMC ea mohato o le mong
E felisa tlhokeho ea centrifugation ea khale ea "density density gradient centrifugation" (mohlala, Ficoll-Paque™).
E fokotsa nako ea ho sebetsa le ho fetoha.
Bophahamo ba mali bo lekantsoeng
E etselitsoe ho sebetsa ho fihla ho 8mL ea mali a felletseng.
E hloekile, e sebelisoa hang feela
E sebelisoa bakeng sa lits'ebetso tsa bongaka le tsa lipatlisiso moo ho hlokehang ho itšehla thajana ea boleng bo holimo ea PBMC.
E lumellana le centrifugation
Ho hlokahala protocol e itseng ea centrifugation ho arola li-PBMC ka katleho le likarolo tse ling tsa mali.

Tšebeliso le lisebelisoa:
PBMC ho itšehla thajana bakeng sa lithuto tsa ts'ebetso ea 'mele ea ho itšireletsa mafung (lisele tsa T, lisele tsa B, lisele tsa NK, monocyte).
Flow cytometry, ELISPOT, litlhahlobo tsa cytokine, le litlhahlobo tse ling tsa immunological.
Mafu a tšoaetsanoang le lipatlisiso tsa ente.
Tlhokomelo ea lefu la Autoimmune.
Liteko tsa bongaka le ho sibolloa ha biomarker.

Kamoo e Sebetsang (Ts'ebetso ea Motheo):
1. Bokella sampuli ea mali ka har'a tube ea CPT (tlatsa molumo o khethiloeng).
2. Fetola ka bonolo (makhetlo a 8-10) ho kopanya le anticoagulant.
3. Centrifuge ho latela protocol ea moetsi (ka tloaelo ho pota 1500-1800 RCF bakeng sa metsotso e 20-30 mocheso oa kamore).
4. Liforomo tsa PBMC tsa lera ka holim'a karohano ea gel ka mor'a centrifugation.
5. Ntša lera la PBMC ka hloko u sebelisa pipette bakeng sa lisebelisoa tse tlaase.

Polokelo le Ts'ebetso:
Boloka mocheso oa kamore (15-25 ° C).
Qoba mocheso o feteletseng kapa serame.